Journal: Journal of Neuroinflammation
Article Title: TRIM21 promotes astrocyte-mediated neuroinflammation in experimental autoimmune encephalomyelitis by stabilizing RGMa via K33-linked ubiquitination
doi: 10.1186/s12974-026-03769-4
Figure Lengend Snippet: RGMa is upregulated in MS patients and EAE mice and localizes to astrocytes. A RGMa levels in peripheral blood samples from MS patients (n = 117) and controls (n = 50), as quantified by ELISA. B Schematic depicting the time course of MOG35-55-induced EAE in the murine model. C Clinical scores of EAE mice following immunization with MOG35-55 peptide (n = 12). D Representative Western blot analysis of RGMa protein expression in brain lysates from EAE mice at 28 dpi (n = 5). E Corresponding Western blot analysis of RGMa expression in spinal cord lysates from EAE mice at 28 dpi (n = 5). F Quantitative assessment of RGMa protein expression in brain lysates, normalized to GAPDH (n = 5). G Quantification of RGMa expression in spinal cord lysates, normalized to GAPDH (n = 5). H Immunofluorescence analysis illustrating the spatial distribution and co-localization of RGMa (red) with the astrocyte marker glial fibrillary acidic protein (GFAP, green) in brain and lumbar spinal cord sections between control and EAE mice at 28 dpi (n = 5). Note: IgG isotype controls confirmed staining specificity (Fig. S2). Scale bar, 50 µm. DAPI, 4',6-Diamidino-2'-phenylindole. I Inflammatory cytokine mRNA expression levels of IL-1β, IL-6, and CCL2 in primary astrocytes at 24 hours after lentivirus-mediated overexpression of RGMa or vector control
Article Snippet: After 10 days of induction with MOG35-55 by which time mice gradually exhibited tail weakness, the mice were randomly assigned to receive daily intraperitoneal injections of either Quisinostat (HY-12726, MedChemExpress, USA) at 10 mg/kg or an equal volume of vehicle control for 2 weeks.
Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Immunofluorescence, Marker, Control, Staining, Over Expression, Plasmid Preparation